A switch-on luminescent europium(iii) probe for selective and time-resolved detection of adenosine diphosphate (ADP).

ABSTRACT: Adenosine diphosphate (ADP) is a key product of two essential classes of biological reactions, catalysed by ATPases and kinases. This makes ADP a highly appealing target for supramolecular detection. However, doing so selectively is exceedingly difficult due to ADP's lower overall charge and similar structure to ATP and the need for compatibility with biological media. Overcoming this challenge, here we present a water-soluble, ADP-selective, luminescent europium(iii) probe suitable for use in vitro and in cellular microscopy. This negatively charged Eu(iii) complex binds ADP reversibly and responds by switching on its luminescence, whilst showing minimal interference from ATP, pyrophosphate and a wide range of biological anions. The probe is equipped with two π-conjugated quinolyl-

SUBMITTER: Bodman SE 

PROVIDER: S-EPMC11868914 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature