Construction of Multiplexed Assays on Single Anisotropic Particles Using Microfluidics.

ABSTRACT: Considerable efforts have been made to develop microscale multiplexing strategies. However, challenges remain due to the difficulty in deploying functional objects and decoding high-density signals on anisotropic microcarriers. Here, we report a microfluidic method to fabricate architecture-marked anisotropic particles for performing designable multiplexed assays in a label-free manner. By controlling fluid assembly and rapid in-air cross-linking, the particles are endowed with multiple functional regions and a unique architecture identifier. The marked architecture enables an addressing mechanism that allows the profiling of embedded label-free objects by mapping a well-defined reference architecture onto the target particle. By loading analytes of interest, such as molecular probes or ce

SUBMITTER: Wu Z 

PROVIDER: S-EPMC11868959 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature