3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation.

ABSTRACT: Clonogenicity of organoids from the intestinal epithelium is attributed to the presence of stem cells therein. The mouse small intestinal epithelium is compartmentalized into crypts and villi: the stem and proliferating cells are confined to the crypts, whereas the villi epithelium contains only differentiated cells. Hence, the normal intestinal crypts, but not the villi, can give rise to organoids in 3D cultures. The procedure described here is applicable only to villus epithelium undergoing dedifferentiation leading to stemness. The method described uses the Smad4-loss-of-function:β-catenin gain-of-function (Smad4^KO:β-catenin^GOF) conditional mutant mouse. The mutation causes the intestinal villi to dedifferentiate and generate stem cells in the villi. Intestinal vil

SUBMITTER: Li C 

PROVIDER: S-EPMC11869120 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature