Design and characterization of allantoin-inducible expression systems in budding yeast.

ABSTRACT:

Background

Saccharomyces cerevisiae has been extensively employed as a host for the production of various biochemicals and recombinant proteins. The expression systems employed in S. cerevisiae typically rely on constitutive or galactose-regulated promoters, and the limited repertoire of gene expression regulations imposes constraints on the productivity of microbial cell factories based on budding yeast.

Results

In this study, we designed and characterized a series of allantoin-inducible expression systems based on the endogenous allantoin catabolic system (DAL-related genes) in S. cerevisiae. We first characterized the expression profile of a set of DAL promoters induced by allantoin, and further combined with the galactose-inducible (GAL) system to create a highly respons

SUBMITTER: Wang J 

PROVIDER: S-EPMC11869459 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature