Hypusinated and unhypusinated isoforms of the translation factor eIF5A exert distinct effects in models of pancreas development and function.

ABSTRACT: Hypusination of eukaryotic translation initiation factor 5A (eIF5A) is essential for its role in translation elongation and termination. Although the function of hypusinated eIF5A (eIF5A^Hyp) in cellular proliferation is well characterized, the role of its unhypusinated form (eIF5A^Lys) remains unclear. We hypothesized that eIF5A^Lys exerts independent and negative effects on cellular replication and metabolism, distinct from the loss of eIF5A^Hyp. To test this hypothesis, we utilized zebrafish and mouse models with inducible knockdowns of deoxyhypusine synthase (DHPS) and eIF5A to investigate their roles in cellular growth. Gene expression analysis via RNA sequencing and morphometric measurements of pancreas and β-cell mass were performed to assess

SUBMITTER: Anderson CM 

PROVIDER: S-EPMC11869520 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature