A leucine responsive small RNA AbcR200 regulates expression of the lactate utilization (lut) operon in Acinetobacter baumannii DS002.

ABSTRACT: Noncoding small RNAs are essential for modulating bacterial gene expression, especially under carbon and nutrient-limited conditions. In this study, by using both in silico and molecular hybridization tools, we identified a carbon source responsive small RNA in Acinetobacter baumannii DS002. Expression of corresponding gene, abcR200, located at the intergenic region of omt (O-methyl transferase) and orf72 genes, is under the transcriptional control of a global transcriptional factor, leucine responsive regulatory protein (Lrp). A sequence motif that serves as a target for Lrp was found overlapping the abcR200 promoter (P_abcR200). Chromatin immunoprecipitation demonstrated that Lrp oligomers, formed under low leucine conditions, strongly interacted to the P_abcR200. How

SUBMITTER: Yakkala HN 

PROVIDER: S-EPMC11869524 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature