Activation of Nrf2 pathway by 4-Octyl itaconate enhances donor lung function in cold preservation settings.

ABSTRACT:

Background

Lung transplantation is the primary treatment for end-stage lung diseases. However, ischemia-reperfusion injury (IRI) significantly impacts transplant outcomes. 4-Octyl itaconate (4-OI) has shown potential in mitigating organ IRI, although its effects in lung transplantation require further exploration.

Methods

BEAS-2B cells were used to model transplantation, assessing the effects of 4-OI through viability, apoptosis, and ROS assays. qRT-PCR analyzed cytokine transcription post-cold ischemia/reperfusion (CI/R). RNA sequencing and Gene Ontology analysis elucidated 4-OI's mechanisms of action, confirmed by Western blotting. ALI-airway and lung transplantation organoid models evaluated improvements in bronchial epithelial morphology and function due to 4-OI. ELISA m

SUBMITTER: Gao X 

PROVIDER: S-EPMC11869626 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature