CTCF is selectively required for maintaining chromatin accessibility and gene expression in human erythropoiesis.

ABSTRACT:

Background

CTCF is considered as the most essential transcription factor regulating chromatin architecture and gene expression. However, genome-wide impact of CTCF on erythropoiesis has not been extensively investigated.

Results

Using a state-of-the-art human erythroid progenitor cell model (HUDEP-2 and HEL cell lines), we systematically investigate the effects of acute CTCF loss by an auxin-inducible degron system on transcriptional programs, chromatin accessibility, CTCF genome occupancy, and genome architecture. By integrating multi-omics datasets, we reveal that acute CTCF loss notably disrupts genome-wide chromatin accessibility and the transcription network. We detect over thousands of decreased chromatin accessibility regions but only a few hundred increased regions a

SUBMITTER: Yang X 

PROVIDER: S-EPMC11869676 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature