Copper nanocubes as low-cost enzyme mimics in a sarcosine-sensing reaction cascade.

ABSTRACT: The development of simple, inexpensive, deployable clinical diagnostics could have a global impact on public health by making measurements of patient health status more widely accessible to patients regardless of socioeconomic status. Here, we report a novel biosensor for sarcosine using a colorimetric readout created by a hybrid catalyst system using copper nanocubes and the enzyme sarcosine oxidase. The enzyme catalyzes the reaction of sarcosine to generate H₂O₂, which the copper nanocubes then use as a substrate to create free radicals that convert colorless 3,3',5,5'-tetramethylbenzidine (TMB) to its blue, oxidized form. The sensor showed good substrate affinity for Cu nanocubes and yielded a wide linear response range (0-140 μM) for sarcosine detection, with high

SUBMITTER: Tripathi A 

PROVIDER: S-EPMC11869937 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature