Recovery efficiences on nucleic acid extraction kits as measured by quantitative LightCycler PCR.

ABSTRACT:

Aims

To compare the efficiency of five nucleic acid extraction kits for processing clinical material for the diagnosis of infection.

Methods

Five nucleic acid extraction kits for processing clinical material for the diagnosis of infection were compared for their relative efficiencies in purifying and recovering either viral DNA or RNA from serum samples. Quantitative polymerase chain reaction (PCR) assays for hepatitis B virus (HBV) DNA and hepatitis C virus (HCV) RNA were performed on the Light-Cycler instrument to determine the relative concentrations of the viral nucleic acids recovered by the various protocols.

Results

Large differences between kits in recovery efficiencies were observed for HBV DNA, with those protocols using enzyme digestion in addition to chaot

SUBMITTER: Read SJ 

PROVIDER: S-EPMC1187008 | biostudies-literature | 2001 Apr

REPOSITORIES: biostudies-literature