Molecular MRI of T-cell immune response to cryoablation in immunologically hot <i>vs</i>. cold hepatocellular carcinoma.

ABSTRACT:

Background & aims

Increasing enthusiasm around integrating locoregional therapy with systemic immunotherapy in primary liver cancer underscores the need for non-invasive imaging biomarkers. In this study, we aimed to establish advanced molecular MRI tools for monitoring T-cell responses to cryoablation in murine models, distinguishing between immunologically "hot" and "cold" hepatocellular carcinoma (HCC).

Methods

Immunocompetent 7-10-week-old C57BL/6J and BALB/cJ mice (n = 18 each) received carbon tetrachloride for 12 weeks to induce cirrhosis. Intrinsically immunogenic Hepa1-6 ("hot") and non-immunogenic TiB75 ("cold") cells were orthotopically implanted into C57BL/6 or BALB/c mice, respectively, to generate focal HCC lesions. After one week, animals were randomly assigned

SUBMITTER: Santana JG 

PROVIDER: S-EPMC11870164 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature