Complementary Volume Electron Microscopy-based approaches reveal ultrastructural changes in germline intercellular bridges of <i>D. melanogaster</i>.

ABSTRACT: Intercellular bridges are essential to connect developing germline cells. The Drosophila melanogaster egg chamber is a powerful model system to study germline intercellular bridges, or ring canals (RCs). RCs connect the developing oocyte to supporting nurse cells, and defects in their stability or growth lead to infertility. Despite their importance, it has been technically difficult to use electron microscopy-based approaches to monitor changes in RC structure during oogenesis. Here, we describe the application of a complementary set of volume EM-based approaches to visualize ultrastructural changes in the germline RCs. The combination of array tomography (AT) and focused ion beam (FIB) scanning electron microscopy (SEM) has allowed us to gain insight into previously unappreciated

SUBMITTER: Kolotuev I 

PROVIDER: S-EPMC11870571 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature