High Dimensional Proteomic Multiplex Imaging of the Central Nervous System Using the COMET^™ System.

ABSTRACT: Sequential multiplex methodologies such as Akoya CODEX, Miltenyi MACSima, Rarecyte Orion, and others require modification of the antibodies by conjugation to an oligo or a specific fluorophore which means the use of off-the-shelf reagents is not possible. Modifications of these antibodies are typically performed via reduction chemistry and thus require verification and validation post-modification. Fixed panels are therefore developed due to various limitations including spectral overlap that creates spectral unmixing issues, steric hindrance, harsh antibody removal, and tissue degradation throughout the labeling. As such, a complex interrogation evaluating multiple study hypotheses and/or endpoints requires the development of sequential panels, reconstruction, and realignment of the tissu

SUBMITTER: Najem H 

PROVIDER: S-EPMC11870576 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature