Differentially expressed genes in association with in vitro invasiveness of human epithelioid sarcoma.

ABSTRACT:

Aims

Differential display reverse transcription polymerase chain reaction (RT-PCR) was performed to identify genes associated with the invasive potential of human epithelioid sarcoma.

Methods

Two different clonal subpopulations, GRU-1A and GRU-1B, derived from the same human epithelioid sarcoma cell line GRU-1 and known to differ greatly in their invasive potential were compared by means of mRNA fingerprinting.

Results

Using a set of 10 arbitrary upstream primers and nine anchored oligo-dT primers, 22 candidate gene fragments were identified; differential expression was confirmed in four of these fragments by northern blot analysis. At the mRNA level, apoferritin light chain was predominantly expressed by the highly invasive cell line GRU-1A. In contrast, the mitochon

SUBMITTER: Weber A 

PROVIDER: S-EPMC1187090 | biostudies-literature | 2001 Oct

REPOSITORIES: biostudies-literature