Advanced multi-modal mass spectrometry imaging reveals functional differences of placental villous compartments at microscale resolution.

ABSTRACT: The placenta is a complex and heterogeneous organ that links the mother and fetus, playing a crucial role in nourishing and protecting the fetus throughout pregnancy. Integrative spatial multi-omics approaches can provide a systems-level understanding of molecular changes underlying the mechanisms leading to the histological variations of the placenta during healthy pregnancy and pregnancy complications. Herein, we advance our metabolome-informed proteome imaging (MIPI) workflow to include lipidomic imaging, while also expanding the molecular coverage of metabolomic imaging by incorporating on-tissue chemical derivatization (OTCD). The improved MIPI workflow advances biomedical investigations by leveraging state-of-the-art molecular imaging technologies. Lipidome imaging identifies molecul

SUBMITTER: Velickovic M 

PROVIDER: S-EPMC11871073 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature