Intratracheal instillation for the testing of pulmonary toxicity in mice-Effects of instillation devices and feed type on inflammation.

ABSTRACT:

Background

Inhalation exposure is the gold standard when assessing pulmonary toxicity. However, it typically requires substantial amounts of test material. Intratracheal instillation is an alternative administration technique, where the test substance is suspended in a liquid vehicle and deposited into the lung via the trachea. Instillation requires minimal test material, delivers an exact dose deep into the lung, and is less labor-intensive than inhalation exposures. However, one shortcoming is that the procedure may induce short-term inflammation. To minimize this, we tested different modifications of the technique to identify the potential for refinement.

Methods

First, we tested whether previous findings of increased inflammation could be confirmed. Next, we tested wheth

SUBMITTER: Hadrup N 

PROVIDER: S-EPMC11871123 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature