Fast and efficient method for parallel construction of targeted exome and methylome single-stranded DNA sequencing libraries.

ABSTRACT: Based on single-stranded DNA library method, we established an efficient workflow to parallelly construct targeted genomic and epigenomic sequencing libraries from a small amount of DNA. We applied the protocol to nine pediatric brain cancer DNA samples containing various extents of damage from formalin fixation and/or DNA oxidation. Compared to our previous study, the new exome protocol showed superior uniformity of coverage. Many artifactual mutation calls introduced by DNA damages were eliminated by bioinformatics filtering tools. After filtration, 89.4-97.0% of somatic single nucleotide variant (SNV) calls generated by double-stranded DNA library were reproduced in formalin-fixed paraffin-embedded (FFPE) samples, which was achieved with substantially reduced DNA input amounts (26.7-50n

SUBMITTER: Kim E 

PROVIDER: S-EPMC11871346 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature