Discovery and characterization of an FAD-dependent glucose 6-dehydrogenase.

ABSTRACT: Many patients with diabetes use self-measurement devices for blood glucose to understand their blood glucose levels. Most of these devices use FAD-dependent glucose dehydrogenase (FAD-GDH) to determine blood glucose levels. For this purpose, FAD-GDHs specifically oxidizing glucose among the sugars present in blood are required. Many FAD-GDHs with high substrate specificity have been reported previously; however, their substrate specificity is insufficient as they also react with xylose. Therefore, we aimed to identify FAD-GDHs without xylose reactivity. We screened and obtained a new enzyme from Colletotrichum plurivorum (CpGDH). CpGDH showed high activity to glucose in the presence of electron mediators but low activity to xylose. We prepared the glucose oxidation products using CpGDH and

SUBMITTER: Fujii T 

PROVIDER: S-EPMC11871447 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature