The SET29 and SET7 proteins of Leishmania donovani exercise non-redundant convergent as well as collaborative functions in moderating the parasite's response to oxidative stress.

ABSTRACT: SET proteins are lysine methyltransferases. In investigating Leishmania donovani SET29, we found depletion of LdSET29 by two-thirds did not affect promastigote growth, nor alter the parasite's response to UV-induced or HU-induced stress, but made it more tolerant to H₂O₂-induced oxidizing environment. The deviant response to oxidative stress was coupled to lowered accumulation of reactive oxygen species, which was linked to enhanced scavenging activity. The set29 mutants' response to H₂O₂ exposure was similar to that of set7 mutants, prompting an investigation into genetic and physical interactions between the two proteins. While neither protein could rescue the aberrant phenotypes of the other set mutant, the two proteins interacted physically i

SUBMITTER: Sharma V 

PROVIDER: S-EPMC11871502 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature