TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing.

ABSTRACT:

Aims

To evaluate the expression of transforming growth factor beta1 (TGF-beta1) and fibroblast growth factor 2 (FGF-2) mRNA in stromal cells in response to injury in the presence of either TGF-beta1 or FGF-2. It has been shown previously that heparan sulfate proteoglycans and FGF-2 are present transiently during wound repair in vivo and that an increase in TGF-beta1 mRNA is detected rapidly after injury.

Methods

Primary corneal fibroblasts were cultured to confluency, serum starved, and linear wound(s) were made in medium containing TGF-beta1 or FGF-2. TGF-beta1 and FGF-2 mRNA expression were evaluated using both northern blot analysis and in situ hybridisation. Both dose dependent and time course experiments were performed. Whole eye organ culture experiments were also carr

SUBMITTER: Song QH 

PROVIDER: S-EPMC1187169 | biostudies-literature | 2002 Jun

REPOSITORIES: biostudies-literature