Targeting macrophage to myofibroblast transition by circ_0001103 for subretinal fibrosis treatment.

ABSTRACT:

Purpose

Subretinal fibrosis is an important cause of visual loss in age-related macular degeneration, but its mechanism remains unclear. This study aims to investigate the role of macrophage-to-myofibroblast transition (MMT) in the formation of subretinal fibrosis and assess whether circ_0001103 can regulate the formation of subretinal fibrosis by regulating MMT.

Methods

Subretinal fibrosis was induced in C57BL/6J mice by laser induction. The expression profiles of circRNAs in a choroidal neovascularization (CNV) and subretinal fibrosis mice model were accessed via microarray analysis. MMT was induced by TGF-β1 (2.5 ng/ml, 48 h). Immunohistochemistry was used to assess macrophages (F4/80), MMT (α-SMA) and fibrovascular lesions (collagenI and Isolectin B4) in vivo. The intera

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC11871722 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature