The alternative polyadenylation regulator CFIm25 promotes macrophage differentiation and activates the NF-κB pathway.

ABSTRACT:

Background

Macrophages are required for development and tissue repair and protect against microbial attacks. In response to external signals, monocytes differentiate into macrophages, but our knowledge of changes that promote this transition at the level of mRNA processing, in particular mRNA polyadenylation, needs advancement if it is to inform new disease treatments. Here, we identify CFIm25, a well-documented regulator of poly(A) site choice, as a novel mediator of macrophage differentiation.

Methods

CFIm25 expression was analyzed in differentiating primary human monocytes and monocytic cell lines. Overexpression and depletion experiments were performed to assess CFIm25's role in differentiation, NF-κB signaling, and alternative polyadenylation (APA). mRNA 3' end-focused

SUBMITTER: Mukherjee S 

PROVIDER: S-EPMC11871739 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature