Long-read genome sequencing reveals the sequence characteristics of pear self-incompatibility locus.

ABSTRACT: The S-RNase-based self-incompatibility locus (S-locus) in Petunia species contains 16-20 F-box genes, which collaboratively function in the recognition and subsequent degradation of non-self S-RNases, while distinguishing them from self S-RNase. However, the number of S-locus F-box genes (SFBBs) physically interacted with non-self S-RNases remains uncertain in Pyrus species. Utilizing Pacbio long-read sequencing, we successfully assembled the genome of pear cultivar 'Yali' (Pyrus bretschneideri), and identified 19 SFBBs from the Pyrus S₁₇-locus spanning approximately 1.78 Mb. Additionally, we identified 17-21 SFBBs from other Pyrus and Malus S-loci spanning a range of 1.35 to 2.64 Mb. Based on the phylogenetic analysis, it was determined that Pyrus and Malus SFBBs could be class

SUBMITTER: Gu C 

PROVIDER: S-EPMC11871771 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature