Systems metabolic engineering of <i>Corynebacterium glutamicum</i> for efficient l-tryptophan production.

ABSTRACT: Corynebacterium glutamicum is a versatile industrial microorganism for producing various amino acids. However, there have been no reports of well-defined C. glutamicum strains capable of hyperproducing l-tryptophan. This study presents a comprehensive metabolic engineering approach to establish robust C. glutamicum strains for l-tryptophan biosynthesis, including: (1) identification of potential targets by enzyme-constrained genome-scale modeling; (2) enhancement of the l-tryptophan biosynthetic pathway; (3) reconfiguration of central metabolic pathways; (4) identification of metabolic bottlenecks through comparative metabolome analysis; (5) engineering of the transport system, shikimate pathway, and precursor supply; and (6) repression of competing pathways and iterat

SUBMITTER: Dong Y 

PROVIDER: S-EPMC11872490 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature