Filter-Aided Extracellular Vesicle Enrichment (FAEVEr) for Proteomics.

ABSTRACT: Extracellular vesicles (EVs), membrane-delimited nanovesicles that are secreted by cells into the extracellular environment, are gaining substantial interest due to their involvement in cellular homeostasis and their contribution to disease pathology. The latter in particular has led to an exponential increase in interest in EVs as they are considered to be circulating packages containing potential biomarkers and are also a possible biological means to deliver drugs in a cell-specific manner. However, several challenges hamper straightforward proteome analysis of EVs as they are generally low abundant and reside in complex biological matrices. These matrices typically contain abundant proteins at concentrations that vastly exceed the concentrations of proteins found in the EV proteome. The

SUBMITTER: Pauwels J 

PROVIDER: S-EPMC11872570 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature