Specific Calcium Signal Responses in Human Keloid-Derived Fibroblasts During Cyclical Stretching: Basic Research.

ABSTRACT:

Background

Keloids most commonly develop in the regions where the skin is constantly stretched. Although some keloid-derived fibroblasts exhibit higher single calcium spikes than normal dermal fibroblasts during short-time cyclical stretching, the calcium signal responses to long-time stretching remain unclear.

Methods

This study compared the intracellular Ca²⁺ dynamics induced by cyclical stretching stimuli between the control group (normal dermal fibroblasts) and the keloid group (keloid-derived fibroblasts). Each group was cyclically exposed to a two-dimensional stretch (10% strain). A confocal laser microscope was used to examine intracellular Ca²⁺ for 30 min fluorescently. The fluorescence intensity ratio (Fluo-8H/calcein red-orange) was used to ev

SUBMITTER: Mineda K 

PROVIDER: S-EPMC11872593 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature