Improving the therapeutic profile of MSCs: Cytokine priming reduces donor-dependent heterogeneity and enhances their immunomodulatory capacity.

ABSTRACT:

Introduction

MSCs exhibit regenerative, anti-inflammatory and immunomodulatory properties due to the large amount of cytokines, chemokines and growth factors they secrete. MSCs have been extensively evaluated in clinical trials, however, in some cases their therapeutic effects are variable. Therefore, strategies to improve their therapeutic potential, such as preconditioning with proinflammatory factors, have been proposed. Several priming approaches have provided non-conclusive results, and the duration of priming effects on MSC properties or their response to a second inflammatory stimulus have not been fully addressed.

Methods

We have investigated the impact of triple cytokine priming in MSCs on their characterization and viability, their transcriptomic profile, the funct

SUBMITTER: Valencia J 

PROVIDER: S-EPMC11872697 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature