An integrated transcriptomic analysis unveils the regulatory roles of RNA binding proteins during human spermatogenesis.

ABSTRACT:

Background

RNA-binding proteins (RBPs) have emerged as key regulators in testis development and spermatogenesis, yet a comprehensive understanding of their expression dynamics has been lacking.

Methods

This study leverages published single-cell RNA sequencing (scRNA-seq) data to elucidate the complex expression patterns of RBP genes during postnatal testis development and spermatogenesis. Additionally, it uses bulk RNA-seq data to explore the regulatory impact of RBPs on alternative splicing (AS) in non-obstructive azoospermia (NOA).

Results

We have identified cell-specific RNA-binding protein (RBP) genes in various cell types throughout testis development. Notably, distinct RBP gene clusters exhibit significant differential expression, particularly in Sertoli cells a

SUBMITTER: Xu N 

PROVIDER: S-EPMC11872710 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature