Alterations in neutrophil mRNA profiles in multiple sclerosis and identification of candidate genes for further investigation.

ABSTRACT:

Introduction

Multiple sclerosis (MS) is a chronic and debilitating inflammatory disease of the central nervous system (CNS), characterized by demyelination and neurodegeneration. Emerging evidence implicates neutrophils in MS pathogenesis, particularly through processes like neutrophil extracellular traps (NETs) formation and degranulation, which may exacerbate inflammation and autoimmunity.

Methods

RNA sequencing of peripheral blood neutrophils from MS patients and healthy controls identified differentially expressed genes (DEGs). Pathway enrichment and protein-protein interaction (PPI) analyses highlighted potential biomarkers, validated using reverse transcription quantitative PCR (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA).

Results

Our analysis identif

SUBMITTER: Zhang H 

PROVIDER: S-EPMC11873095 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature