Shotgun and Hi-C Sequencing Datasets for Binning Wheat Rhizosphere Microbiome.

ABSTRACT: Binning is a crucial process in metagenomics studies, where sequenced reads are combined to form longer contigs and assigned to individual genomes. Conventional methods, such as shotgun binning, rely on similarity measurements and abundance profiles across multiple samples. However, cost constraints for sequencing and limited sample collection capacity hinder their effectiveness. High-throughput chromosome conformation capture (Hi-C), a DNA proximity ligation technique, has been adapted to accurately bin metagenome-assembled genomes (MAGs) from a single sample, addressing challenges like chimeric MAGs. In this study, we generated over 190 Gb of metagenomic data from wheat rhizospheres grown in two highly calcareous soils of South Australian region and compared conventional and Hi-C binning

SUBMITTER: Regmi R 

PROVIDER: S-EPMC11873117 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature