Development of a 101.6K liquid-phased probe for GWAS and genomic selection in pine wilt disease-resistance breeding in Masson pine.

ABSTRACT: Masson pine (Pinus massoniana Lamb.), indigenous to southern China, faces serious threats from pine wilt disease (PWD). Several natural genotypes have survived PWD outbreaks. Conducting genetic breeding with these resistant genotypes holds promise for enhancing resistance to PWD in Masson pine at its source. We conducted a genome-wide association study (GWAS) and genomic selection (GS) on 1013 Masson pine seedlings from 72 half-sib families to advance disease-resistance breeding. A set of efficient 101.6K liquid-phased probes was developed for single-nucleotide polymorphisms (SNPs) genotyping through target sequencing. PWD inoculation experiments were then performed to obtain phenotypic data for these populations. Our analysis reveals that the targeted sequencing data successfully divided

SUBMITTER: Zhu J 

PROVIDER: S-EPMC11873169 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature