A cautionary note on the potential pitfalls of using N-terminal truncated CD63 to label small extracellular vesicles.

ABSTRACT: Small extracellular vesicles (sEV) are nanosized vesicles that facilitate intracellular communication. A significant research obstacle is the isolation of sEV devoid of non-sEV contaminants. Immunoaffinity capture with sEV-specific antibodies is an attractive approach to purifying sEV, but it risks disrupting the vesicles during antibody dissociation. Furthermore, immunoaffinity capture may require the modification of EV-specific proteins for the incorporation of tags on the EV surface, with unknown implications on EV production and function. The aim of this study was to investigate whether a previously reported CD63 truncation is efficient for the incorporation of small tags on the extravesicular surface. We therefore conjugated ALFA-tag to N-terminal-truncated CD63, and included nanoluci

SUBMITTER: Sulaiman E 

PROVIDER: S-EPMC11873209 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature