Lossless single-molecule counting to absolute quantify proteoforms.

ABSTRACT: A novel immunoassay, termed Protein Interaction Coupling (PICO), is introduced to deliver clear, reference-free quantification of proteoforms - precise quantification. PICO employs a compartmentalized, homogeneous single-molecule assay with lossless and sensitive signal generation, capable of detecting down to a few molecules per reaction. Additionally, it utilizes a background-free, digital enumeration principle, known as decouplexing. PICO is presented as mathematical theories, providing a theoretical understanding of its chemistry. Consequently, PICO demonstrates precise quantification, as exemplified with recombinant and non-recombinant ErbB2 and multi-tagged peptide rTRX targets, validating quantification against internal and external references in both analytical and cellular matrice

SUBMITTER: Gross T 

PROVIDER: S-EPMC11873291 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature