Longitudinal Glioma Monitoring via Cerebrospinal Fluid Cell-Free DNA.

ABSTRACT:

Purpose

Current methods for glioma response assessment are limited. This study aimed to assess the technical and clinical feasibility of molecular profiling using longitudinal intracranial cerebrospinal fluid (CSF) from patients with gliomas.

Experimental design

Adults with gliomas underwent longitudinal intracranial CSF collection via Ommaya reservoirs or ventriculoperitoneal shunts. Cell-free DNA (cfDNA) was extracted and analyzed using PredicineCARE for cancer variant profiling and/or PredicineSCORE for low-pass whole-genome sequencing.

Results

Five patients (two females and three males; median age, 40 years; range, 32-64 years) underwent longitudinal intracranial CSF collection via Ommaya reservoirs (n = 4) or ventriculoperitoneal shunts (n = 1). In total, 47 CSF

SUBMITTER: Riviere-Cazaux C 

PROVIDER: S-EPMC11873801 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature