Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope.

ABSTRACT: Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations in biological samples, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the diffraction-limited three-dimensional distribution of the orientations and positions of ensembles of fluorescent dipoles that label biological structures. We share a set of visualization, histogram, and profiling tools for interpreting these positions and orientations. We model the distributions based on the polarization-dependent efficiency of excitation and detection of emitted fluorescence, using coarse-grained representations we call orientation distribution functions (ODFs). We apply ODFs to c

SUBMITTER: Chandler T 

PROVIDER: S-EPMC11874040 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature