Sos1 ablation alters focal adhesion dynamics and increases Mmp2/9-dependent gelatinase activity in primary mouse embryonic fibroblasts.

ABSTRACT:

Background

Sos1 and Sos2 are guanine-nucleotide exchange factors for Ras and Rac small GTPases, which are involved in a wide range of cellular responses including proliferation and migration. We have previously shown that Sos1 and Sos2 have different effects on cell migration, but the underlying mechanisms are not clear.

Methods

Using a 4-hydroxytamoxifen-inducible conditional Sos1^KO mutation, here we evaluated the functional specificity or redundancy of Sos1 and Sos2 regarding the control of cell migration and dynamics of focal adhesions (FAs) in primary mouse embryonic fibroblasts (MEFs).

Results

Functional analysis of the transcriptome of primary Sos1/2^WT, Sos1^KO, Sos2^KO and Sos1/2^DKO-MEFs revealed a specific,

SUBMITTER: Liceras-Boillos P 

PROVIDER: S-EPMC11874121 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature