Matrix glycosaminoglycans and proteoglycans in human cornea organoids and similarities with fetal corneal stages.

ABSTRACT:

Purpose

We developed human cornea organoids (HCOs) from induced pluripotent stem cells (iPSCs) where single-cell RNA-sequence (scRNA-seq) analysis suggested similarity with developing rather than mature human corneas. We performed immunohistology to determine the presence of corneal glycosaminoglycans as an assessment of maturity. We undertook a detailed comparison of the HCO scRNA-seq data with a recent scRNA-seq study of human fetal corneas at different stages to gauge the HCO's maturity.

Methods

We generated HCOs from a second iPSC line, NCRM-1, to assess the reproducibility of HCO development. We stained sections from both HCO lines with Alcian blue and picrosirius red to determine deposition of sulfated glycosaminoglycans and fibrillar collagens. We immunolocalized glyc

SUBMITTER: Ashworth S 

PROVIDER: S-EPMC11874135 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature