Spatial single-cell profiling and neighbourhood analysis reveal the determinants of immune architecture connected to checkpoint inhibitor therapy outcome in hepatocellular carcinoma.

ABSTRACT:

Background

The determinants of the response to checkpoint immunotherapy in hepatocellular carcinoma (HCC) remain poorly understood. The organisation of the immune response in the tumour microenvironment (TME) is expected to govern immunotherapy outcomes but spatial immunotypes remain poorly defined.

Objective

We hypothesised that the deconvolution of spatial immune network architectures could identify clinically relevant immunotypes in HCC.

Design

We conducted highly multiplexed imaging mass cytometry on HCC tissues from 101 patients. We performed in-depth spatial single-cell analysis in a discovery and validation cohort to deconvolute the determinants of the heterogeneity of HCC immune architecture and develop a spatial immune classification that was tested for the p

SUBMITTER: Salie H 

PROVIDER: S-EPMC11874287 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature