Tracking proteasome degradation: A cross-organ analysis via intact degradomics mass spectrometry.

ABSTRACT: The proteasome is a multisubunit degradation machinery that is essential for maintaining protein homeostasis by breaking down unnecessary or damaged proteins into peptides. While most of these peptides are further processed into amino acids, a subset evades complete degradation and plays key roles in biological processes such as antigen presentation, signaling, and apoptosis. However, the variability in peptide lengths and the diverse composition of proteasomes make their comprehensive identification and characterization particularly challenging. Here, we present a method that enables real-time identification of generated peptides, as well as uncleaved and partially cleaved protein substrates, revealing the processive nature of protein proteasomal degradation. Our intact degradomics workfl

SUBMITTER: Zittlau KI 

PROVIDER: S-EPMC11874349 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature