CTCF-mediated insulation and chromatin environment modulate Car5b escape from X inactivation.

ABSTRACT:

Background

Genes that escape X-chromosome inactivation (XCI) in female somatic cells vary in number and levels of escape among mammalian species and tissues, potentially contributing to species- and tissue-specific sex differences. CTCF, a master chromatin conformation regulator, is enriched at escape regions and may play an important role in regulating escape, but the molecular mechanisms remain elusive.

Results

CTCF binding profiles and epigenetic features were systematically examined at escape genes (escapees) using mouse allelic systems with skewed XCI to distinguish the inactive X (Xi) and active X (Xa) chromosomes. We found that six constitutive and two facultative escapees are located inside 30-800 kb domains marked by convergent arrays of CTCF binding sites, consiste

SUBMITTER: Fang H 

PROVIDER: S-EPMC11874400 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature