Planar device-enabled speckle illumination for dark-field label-free imaging beyond the diffraction limit.

ABSTRACT: Dark-field microscopy is a technique used in optical microscopy to increase the contrast in unstained samples, making it possible to observe details that would otherwise be difficult to see under bright-field microscopy; thus, it has been widely employed in biological research, material science, and medical diagnostics. However, most dark-field microscopy methods cannot overcome the optical diffraction limit and require a bulky dark-field condenser and precise alignment of each optical element. In this study, we introduce a planar photonic device that can produce random speckles for dark-field illumination and improve the optical resolution. This planar device is made of random distribution fibers for injection of a laser beam, a scattering layer to produce random speckles, a one-dimension

SUBMITTER: Fan Z 

PROVIDER: S-EPMC11874417 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature