Triple Enzymatic Cascade Reaction to Produce Hydroxytyrosol Acetate from Olive Leaves Using Integrated Membrane Bioreactors.

ABSTRACT: An integrated system of three membrane bioreactors (MBRs) has been developed that cascades three different enzymatic reactions. The integrated system was applied to produce hydroxytyrosol acetate from oleuropein extracted from olive leaves. Different reactor configurations for each reaction were tested and individually optimized to select the MBR to ensure high conversion and continuous production of oleuropein aglycone (OA), hydroxytyrosol (HY) and hydroxytyrosol acetate (HA). Based on this study, the most performing configuration of the integrated system was identified. In the first reaction, oleuropein was converted to OA using a biocatalytic membrane reactor (BMR) with immobilized β-glucosidase in polymeric membranes (conversion 95 %). The OA was then fed to another BMR, where it was c

SUBMITTER: Mazzei R 

PROVIDER: S-EPMC11874677 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature