Enhancing the stability of continuous fermentations for platform chemical production.

ABSTRACT: A significant bottleneck of continuous fermentation is the stability of plasmid-based expression systems. This work tested five plasmid addiction systems based on essential gene complementation in continuous E. coli fermentation. The essential genes tested were infA, ssb, proBA, proC, and dapD and evaluated under phosphate-limited continuous fermentation at two dilution rates (0.033 h^-1 and 0.1 h^-1) and two temperatures (30°C and 37°C). Of these, plasmids stabilized by infA, ssb, and dapD complementation were segregationally stable under all operating conditions. While a lower dilution rate decreased structural stability, this could be remedied by lowering the temperature. At 0.033 h^-1 and 30°C,

SUBMITTER: Outram V 

PROVIDER: S-EPMC11875142 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature