Electrophoresis-Correlative Ion Mobility Deepens Single-Cell Proteomics in Capillary Electrophoresis Mass Spectrometry.

ABSTRACT: Detection of trace-sensitive signals is a current challenge in single-cell mass spectrometry (MS) proteomics. Separation prior to detection improves the fidelity and depth of proteome identification and quantification. We recently recognized capillary electrophoresis (CE) electrospray ionization (ESI) for ordering peptides into mass-to-charge (m/z)-dependent series, introducing electrophoresis-correlative (Eco) data-independent acquisition. Here, we demonstrate that these correlations based on electrophoretic mobility (μ_ef) in the liquid phase are transferred into the gas phase, essentially temporally sorting the peptide ions into charge-dependent ion mobility (IM, 1/K₀) trends (ρ > 0.97). Rather than sampling the entire IM region broadly, we pursued these predictable

SUBMITTER: Shen B 

PROVIDER: S-EPMC11875174 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature