A Streamlined Point-of-Care CRISPR Test for Tuberculosis Detection Directly from Sputum.

ABSTRACT: Mycobacterium tuberculosis (Mtb) is a major threat to global health and is responsible for over one million deaths each year. To stem the tide of cases and maximize opportunities for early interventions, there is an urgent need for affordable and simple means of tuberculosis diagnosis in under-resourced areas. We sought to develop a CRISPR-based isothermal assay coupled with a compatible, straightforward sample processing technique for point-of-care use. Here, we combine Recombinase Polymerase Amplification (RPA) with Cas13a and Cas12a, to create two parallelised one-pot assays that detect two conserved elements of Mtb (IS6110 and IS1081) and an internal control targeting human DNA. These assays were shown to be compatible with lateral flow and can be rea

SUBMITTER: Dunkley ORS 

PROVIDER: S-EPMC11875272 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature