Expanding the fluorescent toolkit: Blue fluorescent protein-expressing Plasmodium berghei for enhanced multiplex microscopy.

ABSTRACT: Fluorescent proteins are widely used as markers to differentiate genetically modified cells from their wild-type counterparts. In malaria research, the prevalent fluorescent markers include red fluorescent proteins (RFPs) and their derivatives, such as mCherry, along with green fluorescent proteins (GFPs) and their derivatives. Recognizing the need for additional fluorescent markers to facilitate multiplexed imaging, this study introduced parasite lines expressing blue fluorescent protein (BFP). These lines enable simultaneous microscopy studies of proteins tagged with GFP, RFP, or detected by fluorophore-labeled antibodies, enhancing the analysis of complex biological interactions. Expression of BFP throughout the parasite's life cycle was driven by the robust Hsp70 promoter, ensuring sta

SUBMITTER: Atchou K 

PROVIDER: S-EPMC11875362 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature