Phenolic metabolism in Sarcandra glabra is mediated by distinct BAHD hydroxycinnamoyltransferases.

ABSTRACT: Sarcandra glabra (Chloranthaceae) has an elaborate phenolic metabolism, encompassing various hydroxycinnamic acid esters. This may imply that multiple hydroxycinnamoyltransferases are involved in establishing this spectrum of natural compounds. Five coding sequences from S. glabra, belonging to the superfamily of BAHD acyltransferases, have been amplified from S. glabra cDNA, and the proteins were expressed in Escherichia coli. By assaying the proteins biochemically, the main substrates of these enzymes were identified as p-coumaroyl- and caffeoyl-CoA as donor substrates together with varying acceptor substrates. SgHST mainly forms p-coumaroyl- and caffeoylshikimic acid, but also the corresponding quinic acid esters as well as amides with 3- and 5-hydroxyanthranilic acids. SgHQT1 predomina

SUBMITTER: Bomeke P 

PROVIDER: S-EPMC11875395 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature