A Benchtop Round Window Model for Studying Magnetic Nanoparticle Transport to the Inner Ear.

ABSTRACT:

Introduction

The round window membrane (RWM) presents a significant barrier to the local application of therapeutics to the inner ear. We demonstrate a benchtop preclinical RWM model and evaluate superparamagnetic iron oxide nanoparticles (SPIONs) as vehicles for magnetically assisted drug delivery.

Methods

Guinea pig RWM explants were inset into a 3D-printed dual chamber benchtop device. Custom-synthesized 7-nm iron core nanoparticles were modified with different polyethylene glycol chains to yield two sizes of SPIONs (NP-PEG600 and NP-PEG3000) and applied to the benchtop model with and without a magnetic field. Histologic analysis of the RWM was performed using transmission electron microscopy (TEM) and confocal microscopy.

Results

Over a 4-h period, 19.5 ± 1.9% of

SUBMITTER: Goyal MM 

PROVIDER: S-EPMC11875551 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature