Compartmentalized Sesquiterpenoid Biosynthesis and Functionalization in the Chlamydomonas reinhardtii Plastid.

ABSTRACT: Terpenoids play key roles in cellular metabolism and can have specialized functions. Their heterologous production in microbial hosts offers an alternative to natural extraction. Here, we developed a subcellular engineering approach in the model green alga Chlamydomonas reinhardtii by targeting both sesquiterpenoid synthases and cytochrome P450s (CYPs) to the plastid, exploiting its photosynthetic electron transport chain to drive CYP-mediated oxidation without reductase partners. Nuclear-encoded sesquiterpenoid synthases were expressed with farnesyl pyrophosphate synthase fusions and targeted to the plastid, while CYPs were modified for soluble localization in the plastid stroma by removing transmembrane domains. The plastid environment supported hydroxylation, epoxidation, and oxidation

SUBMITTER: Gutierrez S 

PROVIDER: S-EPMC11875560 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature