Auranofin induces disulfide bond-mimicking S-Au adducts in protein thiol pairs.

ABSTRACT: Auranofin is an inhibitor of human thioredoxin reductase, clinically used in the treatment of rheumatoid arthritis. More recently, it has been shown to possess strong antibacterial activity. Despite the structural dissimilarity and the independent evolutionary origins of human thioredoxin reductase and its bacterial counterpart (TrxB), inhibition of bacterial thioredoxin reductase is often suggested to be a major factor in auranofin's antibacterial mode of action. To test this hypothesis, we attempted to determine the mechanism of inhibition of auranofin for bacterial TrxB in the presence of thioredoxin, TrxB's natural substrate. However, the data obtained in these experiments was not consistent with a specific and exclusive interaction between TrxB and auranofin. Instead, it suggested tha

SUBMITTER: Quadros Barse L 

PROVIDER: S-EPMC11875817 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature